A quantidade de tropoelastina identificada pelos anticorpos não apresentou grandes diferenças entre os valores da camada superficial e os da camada intermediária e profunda. RESULTADO: As camadas intermediária e profunda da prega vocal apresentam valores muito superiores aos da camada superficial, nas colorações histológicas. Realizou-se quantificação colorimétrica das fibras elásticas. A prega vocal foi transversalmente seccionada em nove regiões e três cortes de cada fragmento foram utilizados para a realização das colorações Verhoeff e resorcina-fuchsina de Weighert e para a realização do estudo imunohistoquÃmico. ![]() Por estes critérios dez pregas vocais foram obtidas e selecionou-se, aleatoriamente, uma prega vocal que pertencia a um indivÃduo de 28 anos. MATERIAL E MÉTODO: Foram seguidos os seguintes critérios de inclusão: idade entre 25 e 40 anos, gênero masculino, cor branca, morte causada por ferimento de arma de fogo, menos de doze horas de morte, ausência de intubação traqueal e de trauma na região cervical e que, por análise microscópica, não apresentassem qualquer alteração da mucosa das pregas vocais. ![]() ImunohistoquÃmica como método de estudo das fibras elásticas em prega vocal humana Immunohistochemistry as a method to study elastic fibers of human vocal foldĭirectory of Open Access Journals (Sweden)įull Text Available OBJETIVO: O objetivo deste trabalho é o de verificar a aplicabilidade do método imunohistoquÃmico na quantificação das diferentes formas das fibras elásticas em prega vocal humana.
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